Exact(2)
To test this approach we firstly assembled templates for a series of arbitrary genes from our laboratory.
The assembled templates were stored at 4 °C in storage buffer (1 mM EDTA, 10 mM NaCl, 0.1 % NP40, 0.5 % Chaps, 20%% glycerol, 2 mM DTT) at a DNA concentration of 100 ng/μl, and used within 1 week.
Similar(58)
The assembled template is extracted by the addition of 200 μl phenol/chloroform, and precipitated by the addition of 500 μl 95% ice-cold ethanol.
For each time point in the wavelet transform, we computed the likelihood that it was generated from one of four previously assembled template distributions.
The building blocks are then generated (in vitro transcription or chemical synthesis), and the individual subunits are assembled (templated or nontemplated) into quaternary architectures.
We investigated the annotated functions of the unique core genes of one, preferably fully assembled, template isolate per unique core genome and searched for genes with plasmid or phage related functions.
All sequence data sets were then assembled using templates from each of the 3 BPI3V genotypes.
The discovery of EST-SSRs in the transcriptome of both species, A. duranensis and A. stenosperma, was performed based on the analysis from assembled contig templates.
In field pea, EST-SSR discovery was performed based on analysis of assembled contig templates, of which 2,345 177%) contained at least one repetitive motif.
EST-SSR discovery was performed based on analysis from assembled contig templates, and a total of 2,929 distinct loci were identified, a frequency of 16% (2,415 SSR containing contigs/15,354 total contigs).
Di- to hexa-nucleotide SSRs with a minimum repeat unit size of five (for tri- to hexa-nucleotide) or six (for di-nucleotide) were identified based on the analysis of assembled isotig templates.
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