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Separate Velvet assemblies were also run with a fixed low and high word length, and the best assembly was chosen from the three trials based on the total assembled length of contigs.
After sequencing of all the libraries was complete, we analyzed all of the sequences together to produce a single assembly consisting of 56,218 unigenes averaging 625.6 bp in length, for a total assembled length of 35,168,415 bp.
The assembled length of pseudomolecules in our MG-20 reference covers 67% of estimated genome size (Sato et al. 2008), which also influences read mapping output.
Table 1 shows the transcriptome sequencing and assembly statistics, 79.66% of the reads were assembled into 29,911 contigs, and then further into 24,014 isotigs, with an average assembled length of 1,638 bp.
With the addition of the initial small scaffolds, the total assembled length of the draft genome presented here is 412,253,695 bp, 76.5% of the predicted pennycress genome size of 539 Mb.
The ESTs were assembled into 20,190 contigs and 36,028 singletons for a total of 56,218 unique sequences and a total assembled length of 35,168,415 bp.
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Meanwhile, the unmapped contigs cover approximately 32.9 Mb of total assembled length.
In their study, 38,159 transcripts were annotated with an average assembled sequence length of 678.65 bp as compared to our results of 44,448 annotated transcripts with an average length coding length of 1,228.27 bp.
The total assembled sequence lengths of the 36 isoforms ranged from 503 to 2,460 bp and only 14 contained complete open reading frame (ORF).
Predictably, the number of assembled sequences and the length of assembled contigs increased with decreasing match percentage and decreasing match size (Table 1).
Table 1 represents the assembly information, including the final cleaned reads for the assembly, mRNA count for each species, assembled consensus sequence and average length of assembled consensus.
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