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Unitigs are contigs that can be assembled in a unique way, and thus repeat and unique regions are assembled into separate unitigs.
Following sequence analysis of SAGE libraries, data were assembled in a unique matrix.
To maximize the utility of these collections, ESTs from all of these efforts, together with those in public databases (e.g. [ 26]), have been assembled in a unique public database: the GigasDatabase http://public-contigbrowser.sigenae.org:9090/Crassostrea_gigas/index.html containing 29,745 unique sequences.
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Singletons are defined as unique sequences that could not be assembled in a cluster whereas singlets are unique sequences that were assembled in a cluster but could not be assembled in a contig.
The remaining contigs of the six sets were further assembled, using Minimus 2 with REFCOUNT = 0 and MINID = 90: in a first step, the six sets of contigs were assembled two by two (split and unsplit), to produce three sets of sequences; in a second step, the three sets were assembled into a unique set of assembled sequences.
The steroid receptor RNA activator (SRA) is a unique modulator of steroid receptor transcriptional activity that functions as a regulatory RNA assembled in a ribonucleoprotein complex.
Cardboard assembled in a quasi-haphazard fashion.
Cells were assembled in an Ar atmosphere.
These ESTs were assembled in 12,709 unique transcript sequences and nearly 4,000 sequences were functionally annotated.
The fragments scFv-gpD and gpD-GFP were assembled in the unique gene encoding for the scFv-gpD-GFP by 20 cycles of PCR-like amplification without primers.
The 103,000-probe SFP-discovery oligonucleotide array used in this study was built from a relatively small EST data set for current standards: 88,000 phred-20 filtered 5'-Sanger-sequenced ESTs assembled in 21,428 unique sequences.
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