Exact(6)
For this purpose we assembled expression data from publicly available oligo-array [10] and MPSS (Massively Parallel Signature Sequencing) experiments [11].
The second data set is from a previously assembled expression compendium of chromatin modifiers [ 13].
The assembled sequence did not contain mutations in any of the assembled expression cassettes.
Correctly assembled expression plasmids were identified by restriction digestion and DNA sequencing (MWG Biotech, Ebersford, Germany) of the entire coding sequence.
We assembled expression data from three studies in which raw data had been deposited in Gene Expression Omnibus (GSE13355, GSE14905 and GSE30999; Affymetrix Human Genome Plus 2.0 array; see Methods) [ 8, 11, 19].
Thus, the concept of transient ES attenuation rather than immediate and complete ES silencing may be of some physiological relevance; it could allow probing for ES performance, for example ESAG functionality, VSG switching orders, quality of novel mosaic VSGs, or newly assembled expression sites.
Similar(54)
These assembled expressions are executed with the help of Runge-Kutta scheme with MATLAB.
The problems, goals, causal and final assumptions and side-effects were extracted from the assembled expressions.
This led us to conclude that analyzing assembled transcript expression profiles was essentially interchangeable with analyzing annotated transcript expression profiles.
The first was an expression-PLN, derived from a transcriptional correlation network assembled using expression levels of the yeast genes measured along a cell-cycle [12].
The synthesized cocoa genes and cocoa gene combinations were assembled in expression cassettes using strong constitutive promoters and ligated into plasmid pBS01A, which resulted in 14 different plasmids (Additional file 1: Table S2; Fig. 2).
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