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Sequences were assembled and checked for read errors and hypermutation [27].
Consensus sequences were assembled and checked for ambiguities by using SeqMan version 8.0.2 (DNASTAR, Madison, WI, USA).
Subsequently, the PCR amplicon sequences were assembled and checked against the transcript sequences annotated in Ensembl 56.37a.
For each genome, overlapping sequence fragments were assembled and checked for ambiguity using Sequencher™ 4.2.2 (Gene Codes Corp).
Sequences were assembled and checked manually using the CLC Main Workbench (http://www.clcbio.com/) and searched against the NCBI nr database by using BLASTn (18).
Sequences were assembled and checked using the base-calling software of the respective sequencers, and then aligned using ClustalX v. 1.81 [ 59].
Similar(53)
In these cases, the EST clusters were re-assembled and checked manually to eliminate errors.
Microsatellite data were scored in GENEMAPPER v.3.7 (Applied Biosystems) and sequence data were assembled and manually checked in GENEIOUS v.4.7.6 (Biomatters, Auckland, New Zealand).
assamica, C. oleifera and C. taliensis[ 18] were used in our study with a minor revision by the two following steps: 1) assembled and manually checked more carefully; 2) PCR were used with high fidelity polymerase to verify the sequences in the four junctions.
Sequence fragments were assembled into contigs, and checked for errors and ambiguities against chromatograms using Sequencher™ 4.6 (Gene Codes).
Engineer, assemble, and check equipment properly: Ensure that valves, manifolds, piping, lines, and other equipment is designed and rated for the full pressure and temperature range of operation.
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