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To gain insight into molecular mechanisms underlying the guppy's sexual dimorphism, we assembled a reference transcriptome combining genome-independent as well as genome-guided assemblies and analyzed sex-biased gene expression between different tissues of adult male and female guppies.
We constructed and sequenced an mRNA library of the High Five cell line, assembled a reference transcriptome for function and expression studies.
In this study we assembled a reference transcriptome from representative natural populations of F. grandis collected from Gulf Coast estuaries ranging from Texas to Florida.
We assembled a reference reaction data set containing reactions assigned with KO for each KEGG map (see Additional file 1: Figure S1).
To provide global view of the transcriptional changes between the ten pearl oyster gonadic categories, we assembled a reference gonad transcriptome de novo.
We assembled a reference transcriptome consisting of 25,002 isogroups (unique gene models) and 102,623 singletons from 2.76 million 454 and Sanger cDNA sequences from coastal Douglas-fir.
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DNA barcoding in the current global sense refers to an international programme to assemble a reference library for biological diversity based on a single target sequence for animals, the cytochrome c oxidaze subunit 1. eDNA refers to trace material left behind in the environment, e.g. from hairs shed or cells left in faeces.
Already these results can be used to assemble a reference map of the brain.
We first combined the reads from all libraries to assemble a reference transcriptome for annotation purposes.
Here we extend these resources by assembling a reference transcriptome using high depth Illumina sequencing.
We used Newbler v2.3 (Roche GS De Novo Assembler) to assemble a reference transcriptome from 454 and Sanger sequences.
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