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The DBC self-assembled efficiently to form aggregates that encapsulated small molecules such as nile red and pyrene.
Together these results indicate that this aptamer-based nanostructure assembles efficiently, that it is modular and interchangeable with respect to individual components, and that the light-up properties of 3WJdB reveal retention of productive RNA fold upon assembly.
In the present study, some cysteine residues were introduced at the N-terminus of calmodulin by genetic engineering, and the resulting engineered calmodulin could be assembled efficiently with oriented manner.
Similar (heteromeric) P2X subunits also formed trimers, as shown for co-expressed P2X1 and P2X2 subunits, which assembled efficiently to a P2X1+2 receptor that was exported to the plasma membrane.
Finally, protofibrils self-assemble efficiently into large mature amyloid fibrils, perhaps through association of monomers or oligomers, which is often accompanied by a structural reorganisation of aggregates [1].
There is no easy way to co-assemble efficiently these two data sets, so we devised a method that assembles the genome first using the longest reads, then corrects the remaining errors by aligning the shortest sequences to the consensus.
(55) Catenane (interlocking ring-like structures) was assembled efficiently without enzymatic ligation.
The NVCP yield was ~340 μg/g FW in the leaves and the protein assembled efficiently into NVLPs [ 55].
The second protein that the reviewer alludes to (the RD-EspP chimera) is assembled efficiently in vivo.
However, Figure 4A shows that a trimeric complex containing only Rio2-Ran-GTP and Crm1 assembles efficiently in the absence of Slx9.
Intact GFAP remained largely (95%) in the supernatant fraction and it had assembled efficiently (Supplementary Figure S2 available at http://www.asnneuro.org/an/005/an005e125add.htm).htm
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