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Alternatively, the derived transcripts may be from the cDNA of untranslated regions, chimerical sequences (assemblage errors) and non-conserved areas of proteins where homology is not detected, which is in agreement with several other transcriptomic studies [43], [46], [47].
Also, it is important to know that current popular genome sequencing and assembling strategies may cause assemblage errors for those highly similar multicopy genes, especially for 16S rRNA gene.
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However, in reality, clearances in joints are unavoidable due to assemblage, manufacturing errors and wear.
While aggregation error could confound conclusions drawn from such comparisons, a related NaGISA study on polychaetes [29] similarly found evidence that nutrient contamination and pollution can have an effect on such an assemblage at continental and intercontinental scales.
Only copies flanked by perfect 5 bp-duplication target sites that result from repair of the integration event were considered, in order to eliminate chimerical copies that could have resulted from errors introduced during genome assemblage.
The assemblage winter temperatures were estimated to be 24.3 °C using both techniques (FP-12E error; ±2.48 °C, SIMMAX-28 error; ±1.27 °C, Table 2).
Layer 3, in turn, was sampled from squares M13-14, arearea where layer 2 was almost sterile and, therefore, the possibility of contamination of the layer 3 faunal assemblage by later material (e.g., as a result of decapage error) could be excluded.
Alternatively, this may be some relative measure of error in the spatial and/or temporal grouping of localities in an assemblage.
Taking into account the margins of error of SIMMAX-28 (±1.27 °C) (Pflaumann and Jian 1999) and FP-12E (±2.48 °C) (Thompson 1981), the February assemblage SSTs are inconsistent with the observed SST (17.7 °C), as they were 4.9 and 2.6 °C warmer than the observed SST, respectively.
Our results reveal that in each of the two regions, the oldest interacting clades are indeed of matching age, at least within the error of molecular clock dating and given our incomplete species sampling, but the North American bird/plant assemblage is roughly half as old as the temperate South American one, yet has more than 3x as many bird-pollinated plant species.
One of his specialties was assemblage.
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