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For characterization of the hydrolytic activity of HoBGLA using cellobiose and lactose, the glucose oxidase (GOD) and horseradish peroxidase (POD) assays were used as described by Kunst and coworkers (Kunst et al. 1988).
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This assay was used as described by the manufacturer.
Primers and PCR conditions were used as described by Marciniak et al. [30].
were used, as described by Wang et al., recently.
Both kits were used as described by the manufacturers.
Clonal density was used as described by Patrawala et al.
Methods were used as described previously [8].
Protocols were used as described before [13].
were used as described [ 19].
A standard western blotting protocol was used, as described previously39.
FluoroJadeB was used as described [24], [28].
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