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Test compounds were diluted in DMSO, and assays were run per the manufacturer's instructions.
PCR assays were run in triplicate, and expression data were averaged.
Invasion assays were run for 6h.
12 Samples were blinded and assays were run daily.
All biochemical assays were run in triplicate.
Assays were run on a microplate reader (Multiskan Ascent, Thermo Electron Corporation, Vaanta, Finland).
All enzyme assays were run in triplicate and average activity was calculated.
All cytotoxicity assays were run at 50 1 and 25:1 effector/target (E/T) ratios.
Cytokine assays were run as manufacturer instructed.
The assays were run in triplicate.
Viral neutralization assays were run as previously described [45].
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CEO of Professional Science Editing for Scientists @ prosciediting.com