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PKT assays were recorded using a cell-screening microscope [24] equipped with a laser autofocus device [25].
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After incubation at 37 °C with 5% CO2 for 1 h, the absorbance at 490 nm, which is known to correlate with the cell number in the assay, was recorded using an ELISA 96-well plate reader.
Readings were recorded using a Bio-Rad enzyme-linked immunosorbent assay reader at 490 nm, with subtraction for plate absorbance at 530 nm.
Light units were recorded using a luminometer.
Injuries were recorded using a structured questionnaire.
Single measurements of each well were recorded using an integration time of 200 ms. Two different versions of the Quantigene® bDNA assay (v1.0 and v2.0) were used.
Following the addition of 50 μl/well Nano-Glo Luciferase Assay Substrate (Promega) light emission was recorded using a BMG Labtech Fluostar plate reader and data analysed as in Section 2.5.
The assay lasted a total of 20 min. Behavior during the session was recorded using a camera mounted above the apparatus and analyzed recording using Mediacruise recording software (Canopus).
Luminescence was recorded using a plate reader.
Fish behavior was recorded using a webcam.
The absorbance at 490 nm was recorded using an enzyme-linked immunosorbent assay (ELISA) plate reader.
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