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Antiviral assays were performed utilizing TZM-bl cells as previously described [13].

ChIP assays were performed utilizing the ChIP-IT Express kit from Active Motif according to the manufacturer's protocol.

Subsequently, invasion assays were performed utilizing basement-membrane coated inserts which separate the cells from medium with 20% fetal calf serum (FCS) in the lower well.

Assays were performed utilizing a progesterone antibody (SAPU R7044X) previously validated for radioimmunoassay (Campbell et al., 1998) in combination with a Progesterone-3-Horse Radish Peroxidase conjugate obtained from Abiox plc (Newberg OR, USA).

Cells were serum-starved for 5 h in 1% FCS and invasion assays were performed utilizing basement-membrane coated inserts which separate the cells from medium with 20% FCS in the lower well as described in Figure  5D.

The phagocytosis assay was performed utilizing the human monocytic cell line THP-1 or human mononuclear cells.

An adenylate kinase non-destructive cytotoxicity assay was performed utilizing the ToxiLight bioassay kit (Lonza) following the manufacturers standard protocol for adherent cells in a 96 well plate.

Animals were sacrificed and tissues collected and processed as described for immunohistochemistry. TUNEL assay was performed utilizing a kit for in situ detection of apoptosis, Oncogene, (Cambridge, MA), according to manufacturer's instructions.

An electrophoretic mobility shift assay for NF- κB was performed utilizing the gel shift assay kit from Promega (Madison, WI, USA), as described previously [ 41].

These assays were performed using the same primer pairs utilized to determine element copy number abundance via quantitative PCR (see Materials and Methods).

Proliferation assays were performed with the Promega CellTiter 96 Assay.