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Assays were obtained from Applied Biosystems, Darmstadt, Germany.
PBMC used as effector cells in the ADCC assays were obtained from Dr. Marjorie Robert-Guroff.
C. elegans N2[wild-type] and GE24[pha-1 e2123)], E. coli food sources OP50 and HB101 (OP50 for compound efficacy and aging assays and HB101 for sensitized and food clearance assays) were obtained from the Caenorhabditis elegans Genetics Center.
Antibiotic disks for solid assays were obtained from Bio-Rad; powdered drugs for liquid assays and soft agar overlays were obtained from Sigma and diluted to the desired concentrations in sterile water.
PamChip® Tyrosine kinase microarrays and BioNavigator software for analysis of peptide microarrays were obtained from PamGene International BV. 10× PK kinase buffer and 100× BSA for kinase assays were obtained from New England Biolabs while ATP was obtained from Sigma.
For quantitative (real time) RT-PCR, specific TaqMAN gene expression assays were obtained from Applied Biosystems which included those for Arginase1 (Mm01190441_g1), Chi3l3 (Mm00657889_mH), Fizz1 (Mm00443109_m1), NOS2 (Mm00440488_m1), Gus-β (Mm00446953_m1), CD206 (Mm01329362_m1) and Real time RT-PCR was performed on cDNA using TaqMAN assay.
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Potato root exudate for use as an attractant in chemotaxis assays was obtained from tissue culture plantlets of potato cv.
MSP119 used in these assays was obtained from the London School of Hygiene and Tropical Medicine (London, UK).
Koda used for the assays was obtained from the PlantiCo Horticulture Breeding and Seed Production, Gołębiew, Ltd. (central region of Poland).
The microorganisms used in the antimicrobial assay were obtained from the American Type Culture Collection (ATCC).
Reagents and antibodies used in ChIP assay were obtained from Upstate.
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