Serial anti-C1q assays were obtained in 11 of the 15 nephritis patients.
The best results across assays were obtained for C. sake CBS 5093, with high sugar consumption rates, associated to low ethanol yields, and very low acetic acid production.
Assays were obtained from Applied Biosystems, Darmstadt, Germany.
PBMC used as effector cells in the ADCC assays were obtained from Dr. Marjorie Robert-Guroff.
Similar results with these assays were obtained with all other cell types examined, including rat lung fibroblasts (RLF), monkey kidney fibroblasts (Cos-1), and mouse fibroblasts (3T3).
pcDNA3-GAL4dbd-Gro/TLE1 plasmids used for transcription assays were obtained by PCR amplification of the entire coding sequence of each mutant using the appropriate pCMV2-FLAG-Gro/TLE1 plasmids as templates.
C. elegans N2[wild-type] and GE24[pha-1 e2123)], E. coli food sources OP50 and HB101 (OP50 for compound efficacy and aging assays and HB101 for sensitized and food clearance assays) were obtained from the Caenorhabditis elegans Genetics Center.
Antibiotic disks for solid assays were obtained from Bio-Rad; powdered drugs for liquid assays and soft agar overlays were obtained from Sigma and diluted to the desired concentrations in sterile water.
PamChip® Tyrosine kinase microarrays and BioNavigator software for analysis of peptide microarrays were obtained from PamGene International BV. 10× PK kinase buffer and 100× BSA for kinase assays were obtained from New England Biolabs while ATP was obtained from Sigma.
Overall, consistent results by triplicate qPCR assays were obtained for 82.9% of DNA sample measurements (n = 2,067/2,493) including 31.3% of sample measurements that had no target bacterial DNA detected (n = 780).
For quantitative (real time) RT-PCR, specific TaqMAN gene expression assays were obtained from Applied Biosystems which included those for Arginase1 (Mm01190441_g1), Chi3l3 (Mm00657889_mH), Fizz1 (Mm00443109_m1), NOS2 (Mm00440488_m1), Gus-β (Mm00446953_m1), CD206 (Mm01329362_m1) and Real time RT-PCR was performed on cDNA using TaqMAN assay.
