Sentence examples for assays were generated using from inspiring English sources

The mutants for biochemical assays were generated using QuickChange XL site-directed kit (Stratagene, La Jolla, CA) and the proteins were purified by the same protocol as for the wild type.

Barcode labels for the semiautomated RNAscope VS Assay were generated using an E-Bar II barcode slide label system (Ventana Medical Systems).

A biotin-labelled probe for the magpie reovirus in-situ hybridisation (ISH) assay was generated using first round pan-reovirus PCR as the template.

Transgenic strains for assaying expression were generated using standard injection techniques with 10 100 ug of test plasmid and 50 ug of the selectable dominant rol-6 plasmid pRF4.

Ligation targets for assay optimization were generated using 500 pg extracted genomic DNA as PCR template.

For calibration of the FasL and Fas TaqMan™ assays, two RNA standards were generated using an in vitro T7-polymerase transcription system (RiboMAX™ Large Scale RNA Production System; Promega, Madison, WI, USA).

To validate the copy number variation assay, artificial template controls were generated using the PCR genotyping amplicons.

Therefore, dose-response curves to doxorubicin and paclitaxel for the parental cell lines as well as cell lines exposed to HIV-PI or doxorubicin for 6 8 months were generated using MTT assays and the IC50 for doxorubicin and paclitaxel for each cell line was determined.

Reporter constructs for assaying enhancer activity and gene expression were generated using Gateway cloning technology (Invitrogen, Carlsbad, CA).

Real-time PCR results were generated using the 5' nuclease assay (TaqMan) and the ABI 7900HT Sequence Detection System (Applied Biosystems, Foster City, CA).

MLVA profiles were generated using a multiplex assay targeting five VNTR loci [ 69].