Exact(11)
Therefore, a subset of the 1,528 assays were generated by selecting 843 assays which have active compounds only (without any inconclusive or unspecified compounds), and the same analyses were performed both for the 1,528 assay set and the 843 assay set.
The RNA transcripts used for the cleavage and binding assays were generated by T7 RNA polymerase using oligonucleotides as templates.
Linear transposon ends for the cleavage assays were generated by PCR using the primers Tn10Armshort and UCTn10 with plasmid pRC915 as template.
Standard curves were generated for each assay to confirm that all assays were generated within acceptable limits (efficiency 93% > × > 107%).
To calculate BAALC and ABL1 copy numbers, standard curves for both assays were generated in every run by 10-fold dilution series of five different plasmid concentrations.
The overall goal for MIATA is to provide the reader with the minimal information necessary to understand how reported data related to T cell assays were generated [ 26].
Similar(49)
Quality checking of the data revealed that almost 30% of SNP assays were generating unreliable or uninformative genotypes.
In this way, an array of sealed cell-based assays was generated without cross-contamination between neighbouring assays.
Prediction networks, based on the ability of assay data to predict the results of other assays are generated and then used to select assay data suitable as a training set for a general cytotoxicity model.
The main MS approach supporting targeted proteomics is selected reaction monitoring (SRM), where specific MS assays are generated a priori and used to selectively detect and quantify proteins of interest in a sample.
A calibration standard for both assays was generated from strain 2993A by cloning a 571-nt genomic fragment into the pGEM-T Easy vector (Promega Corp., Madison, WI, USA).
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