Sentence examples for assays were executed from inspiring English sources
Behavioral assays were executed at variable phases of the circadian clock of the flies.
DNase I and 'in gel' copper-phenantroline (Cu-OP) footprinting assays were executed as described [ 22, 29].
The PCR and Real-Time PCR assays were executed according to the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals [ 25] using oligonucleotides designed on the basis of the differences in the sequences of the fliP gene of B. mallei and Burkholderia pseudomallei.
Screening assays were executed as described by Mukherjee et al. DMSO or compound dissolved in DMSO was added to each assay by pin transfer such that the final concentration of DMSO was 1% (v/v) in each assay.
This is because cells can be incubated and observed within the same microenvironment in which the protein assays are executed.
Table 2 Tyrosinase inhibitory activity of the isolated compounds 1−7 Compounds IC50 (µM a 1 6.7 ± 0.8 2 >50 3 >50 4 2.0 ± 0.1 5 22.0 ± 2.5 6 7.5 ± 0.5 7 >50 Kojic acidb 44.6 ± 0.4 a The assay was executed in triplicate b Positive control used for enzymatic inhibition assay.
A 12-week chronic toxicity assay was executed to investigate the long term toxicity after normal dosage administration of XF and PXF every day.
MTT assay was carried out to measure the cytotoxicity of H2O2, MDA concentration assay was executed to evaluate the degree of oxidative stress, TNF-α ELISA Assay was used to measure the concentration of TNF-α, finally, the degree of necrosis were analyzed using flow cytometry.
Similar assays for effects of LGP2 deficiency were executed for additional genes known to be important for limiting LM growth in the host including IL12p40, tumor necrosis factor alpha (TNFα), and inducible nitric oxide synthase (iNOS) [33], [34], [35].
Assays of [H]-thymidine incorporation were executed as follows: 1.25 × 104 cells (in 100 μl) were seeded in triplicate in 96-well flat-bottom microtiter cell culture plates.
