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Sensitivity and specificity of the assays were evaluated with a blind screening of 95 samples.
In addition, the assays were evaluated with DNA extracted from suspected clinical scab materials obtained from buffaloes, cows and human beings.
Analytical figures of merit and usability of the optimized assays were evaluated with wine and cider as model food processed matrices.
The assays were evaluated with various rotavirus isolates and 89 clinical samples from Virginia, Bangladesh and Tanzania, and exhibited 95% (81/85) sensitivity compared with the conventional RT-PCR-Gel-electrophoresis method, and 100% concordance with sequencing.
Prior to testing in the filtering set, assays were evaluated with manufacturer supplied standard curves to assess limits of detection.
Preamplification efficiencies of all 96 multiplexed assays were evaluated with standard curves, ranging from 5 to 5120 DNA molecules, applying optimal run conditions: 40 nM of each primer, 60°C annealing temperature and 3 min annealing time.
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In this study, the performance of a next generation sequencing (NGS) SNP assay and CE-based STR, mini-STR, and InDel assays was evaluated with a series of fragmented, size-selected samples.
The sensitivity and specificity of the assay were evaluated with 124 clinical bacterial strains, which included 79 methicillin-resistant S. aureus (MRSA), 20 methicillin-sensitive S. aureus (MSSA), 5 Staphylococcus epidermidis, 5 Salmonella Typhimurium, 5 Shigella sonnei, 5 Listeria monocytogenes, and 5 Escherichia coli.
The assay was evaluated with a panel of 13 different flaviviruses.
The assay was evaluated with 100 C. jejuni strains recovered from humans and animals and it was found to be rapid and specific.
Therefore, in the present study, CervicGen HPV RT-qDX (Optipharm), a commercial diagnostic kit targeting a HPV E6/E7 mRNA based on RT-qPCR assay was evaluated with RNA extracted from ThinPrep® Pap samples, and the results were compared to real-time NASBA data.
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