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Pull-down assays were employed to validate the IRs within Pfam domains.
Various assays were employed to analyze and quantify gene induction using fluorescence microscopy of infected cells in culture and histology, and the respective enzymatic assays in vivo using PET and optical imaging.
JC-1 assays were employed to test apoptosis after downregulation of PDGFRs.
To search for DNA DSB, comet assays were employed to examine purified CD4+CD45RO− T-cell populations immediately after isolation and 48+ and 72 h later (Fig 1F).
SDS-polyacrylamide gel electrophoresis and Bradford assays were employed to verify protein purity and to calculate the resulting protein concentration.
To investigate the difference in DNA-binding and transcriptional activation, oligonucleotide selection and electrophoretic mobility shift assays were employed.
CCK-8 and TUNEL assays were employed for apoptosis and cell viability.
Furthermore, the assays were employed effectively to screen 157 germplasm and 159 commercial field samples.
Thermal shift assays were employed to identify potential inhibitors for LmNDK.
To test this hypothesis, two standard fluorogenic assays were employed to report the generation of reactive oxygen species (ROS): singlet oxygen sensor green (SOSG) and hydroxyphenyl fluorescein (HPF).
Agar plate-based assays were employed to assess the antimicrobial activity of these surfaces against Staphylococcus epidermidis.
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