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All assays were done using a protein concentration of 0.5 μM.
Luciferase assays were done using a luciferase assay kit (Promega).
Whole blood assays were done using a methodology similar to that described previously [ 27, 28].
Cell growth/viability assays were done using a modified WST-1 protocol as previously described [ 6].
Microarray assays were done using a service provider (LC Sciences, Houston, USA).
Proprietary miRNA microarray assays were done using a service provider (LC Sciences, Houston, USA) (see electronic supplementary material).
Similar(54)
Invasion assays were done using the Cell Invasion Assay kit (Chemicon International, Inc., CA).
Motility assays were done using tryptone soft agar [ 36].
Yeast assays were done using conventional lithium acetate-based method.
Invasion assays were done using the BD Biocoat Matrigel Invasion Chamber (pore size: 8 μm, 24-well; BD Biosciences, San Jose, CA, USA) following the manufacturer's protocol.
This was done using a scratch assay.
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