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Invasion assays were done using the Cell Invasion Assay kit (Chemicon International, Inc., CA).
Motility assays were done using tryptone soft agar [ 36].
Yeast assays were done using conventional lithium acetate-based method.
All assays were done using a protein concentration of 0.5 μM.
Invasion assays were done using the BD Biocoat Matrigel Invasion Chamber (pore size: 8 μm, 24-well; BD Biosciences, San Jose, CA, USA) following the manufacturer's protocol.
All assays were done using commercially available ELISA, employed according to manufacturer's instructions, with all samples batches analysed at the conclusion of the study and samples from each participant included on the same plate.
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Statistics were done using analyses of variance.
The simulations were done using CST Microwave Studio.
Simulations were done using SIMON and MATLAB softwares.
Stability experiments were done using QC samples.
Statistical analyses were done using SPSS11.5 software.
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