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Superoxide radical scavenging activity, Iron chelation and uric acid inhibition assays were determined using EAF alone based on TAA.
The specificity of multiplex PCR and colorimetric assays were determined using genomic DNA of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Acinetobacter baumannii as negative controls and no alteration was detected.
Statistics for PMN pore formation assays were determined using a one-way repeated-measures analysis of variance (ANOVA) and Tukey's posttest.
The DSe and DSp of the two PCR assays were determined using culture and/or MAT positivity as the gold standard (Table 2).
The statistical significance of differences between EC50 and FC50 values obtained from cell culture drug susceptibility and fusion assays were determined using the Wilcoxon rank-sum test.
Results of protein assays were determined using a Thermomax microplate reader (Molecular Devices, Sunnyvale, CA).
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The significance of the migration assays was determined using 2 Way Anova (Mathematica notebook (Wolfram Research) further developed by Daniel Zicha (Cancer Research UK)).
The significance of the changes in the behavioral assays was determined using the Kruskal-Wallis test.
It should be noted that cell viability in these cytotoxicity assays was determined using trypan blue exclusion.
Statistical significance of the cell proliferation assays was determined using a two-sided Student's t-test.
Relative gene expression levels were computed using β-actin as the internal standard to normalize for variability in mRNA quality, and the amount of input cDNA from two independent assays was determined using the comparative Ct method.
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