Sentence examples for assays were calculated using from inspiring English sources
qPCR reactions were run in duplicate and delta Cts for the splice assays were calculated using the Ct of the pre mRNA qPCR minus the Ct of the mRNA qPCR.
Differences in the distribution of women's characteristics for the three assays were calculated using the Χ distribution.
The cut-off values for the Bio-Rad TeSeE ELISA and Idexx assays were calculated using the mean of the absorbance values of 90 confirmed BSE negative brainstem samples+ 3 standard deviations.
The clinical sensitivity (true positive) and specificity (true negative) of the κ/ λ ratio of N Latex FLC and Freelite FLC assays were calculated using the outcomes of the IFE analysis.
The percentage of growth inhibition for both MTT and SRB assays was calculated using the following formula: % cytotoxicity = A C - A B − A T − A B / A C − A B where AC, AT and AB is absorbance of control, test and blank, respectively.
Sensitivity and specificity of the significant lactate assay were calculated using a cutoff 1.5 times the upper normal range of lactate (2.2 mmol/l) in the two-by-two tables.
Initial angles for the pipette assay were calculated using the pipette location as a reference.
The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of each qRT-PCR assay were calculated using standard procedures.
Statistics for both the melanosome transport and vision startle assay were calculated using the one-way ANOVA paired with the Tukey honestly significant difference test.
The percentage PCR amplification efficiencies (E) for each assay were calculated, using the slope of the semi-log regression plot of cycle threshold versus log input of cDNA (10-fold dilution series of five points), with the following equation: A threshold of 10% above or below 100% efficiency was applied.
