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Statistical significance of the reductions for the colonization and biofilm assays were assessed using a 1-sided t-test of log reductions from at least three experiments per organism.
Rho assays were assessed using a Rhotekin RBD affinity assay as described previously [ 18, 21, 22].
Cholesterol, TG, HDL, sensitive CRP, ALT, AST, and GGT assays were assessed using a C311 Roche analyzer; sensitive CRP immunotubidimetric assays with cholesterol, TG, HDL, ALT, AST, and GGT being enzymatic colorimetic.
The migration assays were carried out using Transwell units (No. 3422; Corning Costar, Tewksbury, MA, USA) and the invasion assays were assessed using the Transwell units coated with Matrigel (No. 354480; BD Biosciences, New York, NY, USA) according to the manufacturer's instructions.
Cell viability assays were assessed using the Cell Counting Kit-8 (CCK-8, WST-8[2- 2-methoxy-4-nitrophenyl -3- 4-nitrophenyl -5- 2,4-disulfophenyl -2 H-tetrazolium, WST-8[2- 2-methoxy-4-nitrophenyl -3- 4-nitrophenyl -5- 2,4-disulfophenyl -2manufacturer's WST-8[2- 2-methoxy-4-nitrophenyl -3- 4-nitrophenyl -5- 2,4-disulfophenyl -2
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Statistical significance of differences in sensitivities between assays was assessed using two-tailed Student's t test.
Statistical significance between groups in the motility and invasion assays was assessed using a Fisher's two-tailed t-test with Microsoft Excel software.
IL-6, ICAM, adiponectin, and insulin analysis (enzyme-linked immunosorbent assays) was assessed using kits supplied by R&D Systems Europe Ltd Oxfordd, UK) and Mercodia AB.
Agreement among ERCC1 expression assays was assessed using Bland-Altman plots and summarised by the concordance correlation coefficient, using the SAS %CCC macro (Barnhart et al, 2002; Crawford et al, 2007).
However, when concordance between the assays was assessed using the adjusted Wallace coefficient, the iPLEX20SNP method (94.9%) was a better predictor of the HRM10SNP method than vice versa (89%).
The specificity and sensitivity of the PCR assay were assessed using genomic DNA from clonal cultures, plasmid copy number of cloned target sequences, as well as from sediment samples.
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