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Quantitative assays were accomplished using a method described previously where 50 100 cells of each construct were randomly scanned and categorized according to their plasma membrane (PM), cytoplasm (C), nuclear (N), or cytoplasm and nuclear (CN) localization patterns.
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The predictions of the mechanism and site of action of the factors were accomplished using a two-factor competition assay.
Measurements of 8-iso-PGF2α and creatinine were accomplished using an enzyme-linked immunosorbent assay (ELISA) method (Cayman Chemical Co, Ann Arbor, MI) without extraction in the urine sample before immunoenzymatic assay.
Docking calculations were accomplished using Glide.
Comparisons were accomplished using a t test for paired values.
These analyses were accomplished using SAS, version 9.3.
All calculations were accomplished using the R framework 3.0.2.
All translations and alignments were accomplished using Geneious software.
All analyses were accomplished using Stata, version 13.1.
Sequence alignments were accomplished using Clustal X 1.81 [ 105].
All the other analyses were accomplished using R3.0.2.
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