Sentence examples for assays utilized from inspiring English sources

But published sensitivities of the assays utilized were not perfect.

The assays utilized either uncloned virus produced in peripheral blood mononuclear cells (PBMCs) (virus infectivity assays, VI assays), or their Env-pseudotyped (gp160) derivatives produced in 293T cells (PSV assays) from molecular clones or uncloned virus.

Aside from differences in the assays utilized, SVR was the defined endpoint in the genome-wide association study (GWAS) identifying rs12979860 [8], whereas null responsiveness to interferon was used in the analysis that endorsed rs12980275 and rs8099917 [10].

This provides additional evidence of retention of low-level enzymatic activity of the 81116AI2 -strain but also provides clues into the biological sensitivity of two of the assays utilized in detection of LuxS enzymatic activity, homocysteine formation using the Ellman reaction and AI-2 activity using the Vibrio harveyi assay.

The degree of Treg depletion was measured by CD4+CD25+ staining for flow cytometric assays on days 1, 7, 14, 21 and 28; these assays utilized dye-coupled monoclonal anti-CD4-phycoerythrin (PE) (clone L3T4; BD Bioscience) and anti-CD25-allophyocyanin (APC) (clone PC61 5.3; Invitrogen, Carlsbad, Ca) antibodies [37].

These results demonstrated that while the inflammatory status of the adipose tissue could be moderately altered by bone marrow-derived JNK1 deficiency, this is insufficient to yield a detectable benefit on systemic insulin sensitivity when the parenchymal cells are intact at the JNK1 locus, under the experimental conditions and assays utilized in this study.

Both assays utilize recombinant soluble attachment glycoproteins (sG) whereas the latter incorporates the cellular receptor, recombinant ephrin-B2.

Calpain cleavage assays utilizing cytoplasmic and nuclear lysates were performed as described above.

Survival assays utilizing siRNA transfected HeLa cells were performed 48 h after the transfection.

We documented the block of CTA1 translocation and CT intoxication with two hour cell culture assays utilizing 100 µM PBA.

The assays utilize the cAMP-Glo Assay kit purchased from Promega (Fitchburg, WI, cat. no. V1501).