Sentence examples for assays utilised from inspiring English sources
Cross-comparing results from different studies are often complicated by the differences in the assays utilised for experiments of this nature (i.e. in vivo versus in vitro enzyme assays) and/or the choice of analytical methods used to generate the data (i.e. degradation of carotenoids versus formation of apocarotenoids).
Table 1 provides a synopsis of the four assays utilised, and their potential problems.
This minireview focuses on many of the basic issues surrounding validation of biomarker assays utilised in clinical trials.
To confirm a direct interaction we first performed a series of in vitro binding assays utilising various recombinantly expressed and purified NCS proteins to act as baits and where either purified recombinant CN or bovine brain cytosol were employed as a source of prey protein.
Our assays utilise thin layer chromatography silica gel sheets and 2, 4 dinitrophenylhydrazine or 4-Benzoylamino-2, 5-dimethoxybenzenediazonium chloride hemi (zinc chloride) salt as the reagents showing a pink or blue product respectively only in the presence ARTs.
Moreover, the 3':5' assays utilise an oligo-dT primer for cDNA preparation, which precludes the cDNA obtained from being used in the majority of RT-PCR assays, an important factor when the amount of RNA is often limited.
These targets may be candidates for assays utilising bromodomain tandem repeats with the addition of a NLS, although the biological relevance of forcing these isolated bromodomains into the nucleus is questionable.
Recently, assays utilising monoclonal antibodies for the measurement of serum free light chains have become available.
To further investigate and characterise the link between Akt1 and integrin αv β3 in transendothelial migration of prostate cancer cells, we performed ECIS assays utilising AP7.4 or LM609.
The bulk of the discussion centres on serological assays utilising the technique of ELISA, since here there is an obvious advantage of sampling multiple time points.
We furthermore compared the binding properties of the recombinant proteins C595scFv-Fc and C595scFv-Fc-IL2 C595scFv-Fc-IL2 C595scFv-Fc-IL2Ab C595 by competition assays utilising dilutions of the fusion prespectivelyof mAb C595, respectovely, that lead tofhalf-maximal binding.
