Sentence examples for assays using the full from inspiring English sources

In the first assays, using the full length purified HIS-SUMO-CP1 and HIS-SUMO-CP4 after refolding, no activity was detected even after long incubation times.

Promoter reporter assays using the full length fascin promoter [ 17] showed that regulation occurred, in both cases, at the transcriptional level (data not shown).

In order to investigate whether or not the SH2 domain of Grb14 is able to bind Tie2 we performed an in vitro pull down assay using the full length Tie2 receptor and the SH2 domain of Grb14.

The binding assays using the full-length and several truncated versions of CRE1/AHK4 (Fig. 2) confirm the hypothesis that trans-zeatin is bound by CRE1/AHK4 via the CHASE domain.

The experimental planning was based on eight assays using the 23 full factorial design combining three important variables for PDT: photosensitizer concentrations, incubation time of cells in HY solutions and employed light dose (λ = 590 ± 10 nm).

The activity of full-length USP11 was assayed using the fluorogenic substrate ubiquitin-AMC.

We performed this assay using the QCM™ Gelatin Invadopodia Assay according to the manufacturer's instructions.

Nuclear run-on assays and transient transfection assays using the rat hepatocyte iNOS full-length promoter and deletion constructs were designed to isolate a cis-acting regulatory element.

The functional relevance of TCF-4 binding to the TBE motive was investigated in luciferase reporter assays using a full-length securin promoter (−1336 to +34).

For the luciferase transactivation assays we used the full-length MMTV long terminal repeat driving transcription of the luciferase gene, pRSV-GR and the human mut or wt BRG1 containing constructs, following the previously described procedures (Johnson et al, 2008).

However, ubiquitination assays using almost full-length HOIL-1L (only lacking a 32-residue C-terminal flexible tail, from now on referred to as HOIL-1L, supplementary Fig S1B online) showed some residual activity after 1 h prompting us to follow the reaction over a longer time period (Fig 1D).