Sentence examples for assays using both the from inspiring English sources
To determine the dynamics of apoptosis induction brought about by the over-expression of the 10 pro-apoptotic proteins, all were analysed in plate-based assays, using both the TUNEL and cleaved-CASP3 assays at 12, 24, 36, 48 and 60 hours following transfection.
Search for CNVs was performed using MLPA assay using both the P348-A2 ATprobeCACNA1A probe mix and P279 CACNA1A probemix (SALSA MLPA Kit, MRC-Holland).
Gradient time of 4 min and column temperature set at 30 °C also allow for the excellent separation of the critical peak pair (RsHNE DNPH/ONE DNPH = 3.3) within only 3.2 min. In order to reveal potential accuracy differences, a series of sample pairs were quantified based on this optimized LC MS/MS assay using both the previously used d3-DNPH and our own [N4]DNPH reagent in AIDA.
The advantages of the single-tube assays using both conventional and quantitative PCR are reduced handling time and prevention of cross contamination compared to regular nested PCR in which the reactions are carried out in two separate tubes.
Chip assays using both promoter regions revealed that FCoR bound the proximal IRE2 but not the distal IRE1.
The generation of memory immune responses was further confirmed by assays using both in vivo antigen challenge and in vitro stimulation.
The effects of the carboxamide N-substituents and the length of the methylene linker have been explored using in silico docking studies, saturation transfer difference NMR spectroscopy and enzyme inhibition assays using both EcDXR and PfDXR.
We reciprocally confirmed PPIs for both MAX/FTH1 (Figure 2B) and SMAD2/RHOA (Figure 2C) with C-terminal labeling pull-down assays using both protein domains and full-length proteins.
Pull-down assays using both ΔN Ncl -GST and C(Ncl)-GST, as well as Stat1-GST, confirmed nucleolin- Stat1 binding in THP-1 cells stimulated for differentiation.
Gel mobility shift and DNase I footprinting assays using both Mor and N-terminal hexa-histidine-tagged α were performed as described previously (Ma and Howe 2004).
Candidate lectins were then verified by lectin-based flow cytometry and immunofluorescent staining assays using both TNBC/non-TNBC cancer cells.
