FLPe assays used the same conditions as Cre assays except that the annealing temperature was raised to 70°C.
As we suspected, the average values obtained for fl-OPN levels were highly variable between assays using the same group of breast cancer patient samples (Fig. 4).
For example, such intuitive knowledge would have predicted small differences in performance between Assay 1 and Assay 2 because both assays used the same primers at the same A&E temperature.
It is relevant to mention that the recombinant protein TvLEGU-1r obtained in this study did not have proteolytic activity and could not be activated (data not shown) for biochemical and direct functional assays, using the same reported conditions [ 58].
The reaction mixtures assayed using the same procedure in the absence of MamK proteins served as negative controls.
In this study, the newly developed triplex real-time PCR assay was compared with a quadruplex conventional gel-based PCR assay using the same primer sets in both assays.
The multiplex RT-nPCR and real-time RT-PCR for detecting BVDV-1 and -2 were developed by Gilbert et al. (1999) and Aebischer et al. (2014b) respectively (Table 1), and these were used to compare with the PNA-AuNP colorimetric detection assay using the same templates on sensitivity.
This assay used the same virus pseudo-particles and the same reagents and was therefore easier to standardize [18].
The reproducibility of the two DArT genotyping arrays was examined by independent assay using the same DNA.
The extracted supernatant was assayed using the same bile acids kit as above after dilution of 1∶5 with PBS.
