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In gene-centered yeast one-hybrid assays, two types of "DNA baits" are used to identify interacting TFs: single copy C. elegans genomic sequences such as gene promoters, and artificial baits such as (putative) cis-regulatory DNA elements [ 5].
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For the enzyme assays three types of controls were used: a stress control (heated cells), a control with no treatment and a negative control consisting of a boiled extract of the non treated cells (inactivated enzyme).
In both assays, the two types of effector cells were the HLA-A*02 01-restricted HLA-A*02 01-restricted HLA-A*02 01-restricted was established from peripheral blood mononuclear cells (PBMCs) of an HCC patient who had received the GPC3144 152 peptide vaccine [ 16], and the HLA-A*02:01-restricted CMV495–503 peptide-specific clonelone, which was established from peripheral healthy volunteer.
Based on a sandwich-type assay format, two types of colorimetric immunoassay protocols were designed and developed for the detection of rabbit IgG (RIgG, as a model) by using the synthesized ISPtNP and conventional HRP as the labeling of detection antibodies, respectively.
We also examined the function of the SNPs associated with CHD susceptibility by performing a reporter gene luciferase activity assay in two types of cell lines.
Infinium I assay involves two types of probes, U and M, complementary to unmethylated and methylated sequence of interest, respectively.
The Infinium I assay uses two types of probes (one for the methylated allele and one for the unmethylated allele), and base extension is the same for both alleles.
As described for BG1Luc assays at UCD, data points >15%RME2 were classified as EA positive for both MCF-7 and BG1-Luc assays at CCi. Figure 5 shows concentration-dependent increases in EA relative to SC at CCi for E2 and a PC extract in 100% EtOH using CCi's MCF-7 or BG1Luc assay and two types of Tritan™ resin pellets stressed by natural sunlight for 14 days or by UVA for 80 hours.
In addition to the standard assay described above, two types of activity measurements were used: the "head space" and "specific activity" assays.
We designed a new GJ intercellular communication (GJIC) assay that used two types of cells (Fig. 1).
The normal reference values for the spectrophotometric assay for the two types of samples were calculated in 26 normal males and are shown in Table 4.
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