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Our study combines high resolution ion conductance measurements with biological susceptibility assays to explore β-lactam translocation properties through Omp36, a major porin of the MDR pathogen, E. aerogenes.
The luciferase assay is one of the most convenient reporter assays to explore the regulation of gene expression in mammalian cell culture.
The need for experimental model systems that can serve as stretch assays to explore fibronectin's mechano-regulated functions motivates the urgency of understanding the structure and properties of the manually pulled fibronectin fibers presented here.
To explore the impact of N-glycosylation on the functionality of AC8, cells expressing AC8, treated with tunicamycin, were tested in in vivo cAMP accumulation assays to explore their regulation by CCE, or in in vitro AC assays (Fig. 3 B ). Tunicamycin had no significant effect on the regulation of AC8 by Ca2+ in vivo or in vitro (Fig. 3 B ).
We view these experiments as intended to explore the biochemical mechanism of CLOCK BMAL1 and CLOCKΔ19 BMAL1 occupancy in a manner analogous to the use of in vitro biochemical assays to explore mechanism in a more simplified state, rather than to recapitulate the complex ClockΔ19 /+ heterozygous state.
In this work, the fluorescent dye PI was used because it increases the sensitivity of cell viability assays to explore chitin synthase function (Ali et al., 2010; Jones and Senft, 1985; Kaloriti et al., 2012; Mascotti et al., 2000; Ramani et al., 1997).
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In this study, the effects of MEGT on an oral cancer cell model were assayed to explore the genotoxic effect, by cell cycle distribution and annexin V detection to explore its apoptotic effect, by γ-H2AX detection to explore its DNA damage effect, by determination of ROS and GSH levels to explore its oxidative stress modifying effect, and by DiOC2(3) staining to explore the MMP depolarization.
Here, we use a new kind of wound healing assay, called a sticker assay, to explore the role of initial wound shape.
The present study was designed to establish a suitable assay to explore CCR2b receptor antagonists from the natural products of Artemisia rupetris and Leontopodium leontopodioides.
Finally, we used the rescue assay to explore whether the selective requirement for endogenous ActRIIA and BMPRII subunits in BMP7-evoked chemotaxis is absolute.
To assess the possibility that Pias3 employs Trim32 as its ubiquitin E3 ligase, we firstly utilized a GST pull-down assay to explore the potential association between Pias3 and Trim32.
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