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Using two independent fluorescence assays, the effect of temperature preconditioning on pathological mPTP opening was tested.
As predicted from cell proliferation and cell cycle assays, the effect was higher in WRO cells (p < 0.01) than in ARO cells (p < 0.05).
In the FIA assays the effect is evidenced by elevated results that require many replications of the analyses, with sample dilutions.
Using previously established steady-state ATP hydrolysis [ 17] and ssDNA filter-binding [ 16] assays, the effect of the same panel of small molecules on each of the three helicases was examined.
Although quenching compounds could yield false positives in both the imaging and total fluorescence assays, the effect of quenching compounds would be limited in the imaging assay, as the potential impact on the spot detection performed by the imaging software is far less than the impact on the actual fluorescence.
Migration of triple-negative MDA-231 breast cancer cells and its modulation by IGFs, the IGF-1R inhibitor NVP-AEW541 and the IGF-2-sequestering monoclonal antibody MAB292 were assessed by the scratch wound healing and Boyden chamber assays; the effect of topotecan (inhibiting hypoxia-inducible factor-1 (HIF-1)) under hypoxia was also evaluated.
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In addition to the motility and biofilm virulence assays, the effects of SIM, LOV and MEV on type 3 toxin secretion and quorum-sensing signaling of P. aeruginosa were also examined.
Using liquid competition assays, the effects of a mismatch repair mutation were detected during the very early stages of growth of a telomerase-defective yeast strain, even before a senescence phenotype was evident (Figure 4).
This protocol is used to assay the effect of protein over-expression on fitness of E. coli.
In this study we have assayed the effect of three different antibiotics on the endosymbiotic microflora of tsetse (Glossina morsitans morsitans).
After 3 months, the four cultures that had the highest content of live cells were selected to assay the effect of As on biomass (As tolerance assays).
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