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Relevantly, Ki-67 assays support a proliferative state of hMSCs on such nanostructured micropatterns comparable to that of standard cell culture platforms, which reinforce the candidature of porous silicon micropatterns to become a conditioning structure for in vitro culture of HMSCs.
Since our previous assays support an important contribution for soluble factors to create an inflammatory protumoral microenvironment, we used MCF-10A cells as sensors of protumoral factors present in media of single and cocultured cells.
At present, DNA- and protein-based assays support both activities but the demand for fast, inexpensive, sensitive methods is increasing.
The results of the two assays support each other except for site 4, site 5 and site 12 that showed high mutagenicity level but low genotoxicity.
Notably, analyses using tissue recombination assays support basal epithelial cells as a cell of origin, whereas in vivo lineage-tracing studies in genetically-engineered mice implicate luminal cells.
Neutralization assays support that although HPV16 L2 epitopes were only exposed in 20-day virions, HPV31 or HPV18 epitopes behaved differently.
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In addition, preliminary in vitro transfection assays supported the interest of these novel bipolar lipid-based lipoplex formulations compared to standard Lipofectamine-based formulations.
The crystal structure of human tRNASec in complex with SepSecS, phosphoserine, and thiophosphate, together with in vivo and in vitro enzyme assays, supports a pyridoxal phosphate dependent mechanism of Sec-tRNASec formation.
Both assays supported the assumption that the light conditions used were indeed causing oxidative stress (data not shown).
In vivo metastasis assays supported this notion (Fig. 4 C and D).
Both investigations found an altered cytokine release in cell-stimulation assays, supporting the view that these SNPs are functionally relevant.
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