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The results below the LLoQ are reported as detected or not detected by the assays software, and such information should not be reported to the clinician because of uncertainty.
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Data were acquired and analyzed using the Sophion Qpatch assay software (Sophion).
Comet tail moments were scored using the CASP software (CaspLab, Comet Assay Software Project, http://casplab.com/download).
PCR was performed on cDNA using one biotinylated primer per pair using sequences adapted from assays designed by PSQ assay software (sequences available on request).
Images were obtained at each set time point and then analyzed to quantify wound healing by using the IncuCyte scratch wound assay software according to the manufacturers' instructions.
The percentages of tail DNA were evaluated using Komet Assay software (Komet version 5.5, Kinetic Imaging Ltd., Nottingham, United Kingdom) under 400× magnification.
Analysis of the HFE codon 282 genotypes was carried out on the PE Biosystems 7700 (Applied Biosystems), using standard allelic discrimination assay software and Taqman Universal PCR Mastermix Applied Biosystemss).
We did obtain quantitative results beyond the assays LLoQs using the Cq and the calibration curve and the manufacturing companies offered some help when the assay software did not produce a value in IVD conditions.
Isobologram analysis was used to evaluate the data from the MTT assays via CalcuSyn software (Biosoft, Great Shelford, Cambridge, UK).
We have designed a system of RUO Quantitative PCR assays and software enabling highly sensitive, stem cell engraftment monitoring.
To handle the high number of generated data, the correlator of advanced real-time assays (CARTA) software was designed to organize samples and to automatically control and analyze TaqMan™ real-time RT-PCR data.
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