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The parameters verified were efficiency of the single PCR assays, precision, input range, and analytical accuracy with respect to reference values.
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Intra-assay precision was 4.2 ± 3.0% and inter-assay precision was 4.5 ± 2.7%.
The relative standard deviation for the assay precision was <7% and the accuracy was >95%.
Linearity, repeatability, inter- and intra-assay precision and accuracy of the method were evaluated.
Intra- and inter-assay precision and accuracy fulfilled the international requirements.
The intra-assay precision and accuracy were in the range of 2.4 4.8 and −1.8%to3.1%1%, respectively.
The inter-assay precision and accuracy were in the range of 3.0 4.2% and −1.2%to3.2%2%, respectively.
The coefficient of variation of the inter-assay precision was less than 11.9%.
Intra-assay precision CV for cortisol was 12.0% to 17.1%; inter-assay precision CV was 4.9%.
Repeatability is also termed intra-assay precision [16].
The assay precision results are shown in Table 3.
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