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On the basis of the affinities at the α1a-, α1b- and α1d-adrenoceptors and the 5-HT1A receptof of a previous series of sixteen 2-[ 2-phenoxyethyl)aminomethyl]-1,4-benzodioxanes ortho monosubstituted at the phenoxy moiety, a number of ortho disubstituted analogues were designed, synthesized in both the enantiomeric forms and tested in binding assays on the same receptors.
The size of the experiment made it impractical, both in terms of time and the number of larvae required, to perform all of the assays on the same day.
The relative abundance of silver stained actin was validated by western blot assays on the same tissue extract (Fig. 1).
We then performed PCT and lactate assays on the same samples by technicians who were blinded to EAA results and clinical diagnosis.
Our 450K samples showed high concordance with WGBS assays on the same DNA, highly preserving their relative position to each other within the first multidimentional scaling (MDS) dimension.
The assay compared well with orthogonal endpoint cell viability assays and additionally provided data at multiple time points and the opportunity to multiplex assays on the same cells.
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To avoid any plate effect, females of MRS and Kisumu were assayed on the same 96-well plates.
Assays employed were commercially available enzyme-linked immunosorbent assay conducted following manufacturer instructions, with all samples batch analysed upon study completion and samples from each participant assayed on the same plate.
Both time points for each subject were always assayed on the same plate.
β-actin was then assayed on the same membrane by using anti-β-actin antibody (1∶1000) and HRP-conjugated IgG.
Gene expression data for other normal tissues assayed on the same platforms were also obtained from GEO.
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determinations on the same
methodologies on the same
tests on the same
essays on the same
analyses on the same
replicates on the same
experiments on the same
concentrations on the same
analysis on the same
assignments on the same
assays on the insect‐cell-expressed
assays on the stromal
assays on the adrenal
assays on the NF-Y-dependent
assays on the dynamic
assays on the red
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