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Even though some molecular functions are enriched for RCPs [ 14], a loss of conservation can make the detailed genetic or biochemistry assays of the function of the AARs difficult.
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At that point, we decided to generate primary mouse fibroblasts (pMEFs) from double transgenic E13.5 embryos and assay for the function of the "Leu3p-α-IPM" system ex vivo.
Future advances are likely to require similar assays for the functions of earlier components in the pathway and will enable us to define their order of assembly, affinities, and specific contributions to the process.
Taken together, results suggest that this newly developed drug Stroop fMRI task may be a sensitive biobehavioral assay of the functions recruited for the regulation of responses to salient symptom-related stimuli in drug-addicted individuals.
The development of assays to evaluate the function of human CD4+CD25+ Tregs in vitro has provided the opportunity to analyze the role of Tregs in human autoimmune diseases such as rheumatoid arthritis (RA).
Thus, such in vitro assays that characterize the function of mutant CaSRs may provide a personalized medicine approach to the selection of appropriate targeted therapies for specific ADH1-causing CaSR mutations.
To clarify the aggressive behaviour of tumours with MCAK overexpression, we used in vitro assays to analyse the function of MCAK in cancer cells.
We used genetic nondisjunction assays to evaluate the function of MEI-S332S124,5,6-D in both meiotic divisions in male meiosis (Table 1).
Gudjonsson and colleagues [ 16] took advantage of these findings and used the behavior of human luminal cells in collagen I as a means of assaying the function of myoepithelial cells in lumen formation.
Furthermore, addition of ascorbic acid and TMPD to assay the function of complex IV revealed a smaller increased of oxygen consumption in MDA231 cells compared to BT474 cells.
Therefore, it was desirable to develop an ORF57-specific functional assay to test the function of PYM in ORF57-mediated translational enhancement, without effecting cellular translation.
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