Assays of self-regulation and obesogenic eating behaviors using behavioral tasks and self-reports are implemented and evaluated to determine feasibility and psychometrics and to test intervention effects.
These cancer stem cells are functionally defined through assays of self-renewal and serial xenotransplantation assays in rodent models.
In the present study, SP cells displayed tumor sphere-like growth, the classical in vitro assay of self-renewal potential, thereby confirming previous findings that SP cells display stem-like activity [34].
Sphere generation is an in vitro assay of self-renewal potential.
These results were consistent with the findings of the in vitro assay of self-renewal potential (Fig. 3; Fig. 4).
The assay of self-incompatible genes S3, S7, and S8, and self-fertile gene Sf was conducted as described by Channuntapipat [ 6].
Since both NOTCH pathway activation and Sox2 levels were upregulated in CD44+CD24low+ compared to CD44+CD24neg, the relationship between them and their importance to sphere-forming ability, a proxy assay of self-renewal was further investigated.
Twelve clones from each integration were assayed for self-activation of HIS3 and lacZ expression and the clone with the lowest level for each bait was used for subsequent screens.
To determine whether Cx46 was essential for tumor initiation, an in vivo surrogate assay of CSC self-renewal, we transduced CSCs with NT or Cx46 shRNA constructs and evaluated their tumor formation ability by transplanting the cells into the brains of immunocompromised mice.
However, assays for the activity of self-renewal, which not only mimic the niche more effectively but potentially involve mixing back the isolated cells with other cell populations at the onset of the assay (much to the same effect as mixing homogenous basal transcription factors in an in vitro reconstituted transcription reaction), have not yet been employed.
