Sentence examples for assays of human from inspiring English sources

Objective: To evaluate two different assays of human sperm DNA integrity, DNA denaturation (DD) and DNA fragmentation (DF), and to correlate these with standard semen parameters.

Similarly, the same results were also observed not only in the assays of human myeloma cell line RPMI8266 but also in the assays of U266 with purified protein derivative or TTA instead of BCGV (see Supplementary Figures 1 3).

cDNA (45 ng) was used to perform qPCR using the primer/probe mix from the TaqMan® Gene Expression Assays of human VEGFA (assay ID Hs00900055_m1) and CA9 (assay ID Hs00154208_m1) with exon spanning probes, and TaqMan® Universal PCR Master Mix using the 7500 Fast Real-Time PCR system (all Applied Biosystems) according to the manufacturer's protocol.

For visualization in immunofluorescence (IF), flow cytometry (FC), and protein staining in western blot (WB) assays of human and zebrafish microtubule-associated protein 1 light chain-3 (LC3 -I/LC3-II, a polyclonal antibody anti-LC3 -I/LC3-IISignaling Technology, 4108) was diluted 200- (IF) 300- (FC) and 1,000-fold (WB).

The resulting cDNA was used to perform qPCR using the primer/probe mix from the TaqMan Gene Expression Assays of human KIT and ETV1 (with exon-spanning probes) and TaqMan Universal PCR Master Mix using the 7500 Fast Real-Time PCR system (all Applied Biosystems) according to the manufacturer's protocol.

The authors carried out quantitative proteomic and phosphoproteomic assays of human embryonic stem cells to observe cellular events for differentiation cells.

Studies have allowed a comprehensive understanding of the biochemical properties of D1(A12), through the use of assays on human cancer cells.

Herein, we developed a PCR-free and label-free fluorescent strategy for facile, reliable and highly sensitive assay of human telomerase activity from crude cancer cell extracts.

Multiplexed assay of human immunoglobulin G and M (IgG and IgM) antibodies with EDP achieves sensitivities down to 0.07 and 0.33 nanomolar, respectively.

A simple yet powerful polymerase chain reaction (PCR -free strategy for visualized assay of human telomerase activity was rePCR -free thistrategy

In vitro platelet aggregation assay of human washed platelets.