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Assays of function that fail to distinguish these populations are therefore hard to interpret.
In accordance with the recommendations of the Society for Biological Therapy (Keilholz et al, 2002), tetramer-based quantitative assays and ELISPOT assays of function were used to determine T cell responses.
In our case, construction of an auxotroph in the BC17 strain allows conclusions from the spectroscopic studies to be directly compared with experimental data derived from in situ assays of function through kinetic spectrophotometry using an extensive collection of mutant strains.
In pursuit of these objectives, we developed a sequence processing pipeline, where data has been filtered and carefully curated to allow for rational selection of candidate plant virus- human miR interactions to be used for future bench assays of function, in order to better understand cross-kingdom interactions, which may be beneficial or harmful.
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Assays of DPEP function by mutagenesis were consistent with its role as a core motif.
Typing was performed at the HLA-A locus in order to determine the appropriate panel of target cells for assays of TIL function.
Assays of Treg function in vitro were performed using MACS-purified CD4+CD25− T cells as effector cells and CD4+CD25+ cells or retroviral transfected Foxp3 expressing CD4+ T cells as regulatory cells.
An alternative approach is to perform assays of HR function.
Serum was collected for assays of hepatorenal function.
Mutation of these residues coupled with assays of receptor function was used to validate and refine the model.
The liver tissues were excised and used for the assays of liver function markers and antioxidant enzymes.
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