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For example, D'Souza RC et al. [32] coupled assays of changes in protein expression, phosphorylation, protein interactions and transcriptional regulation, allowing a brilliant scheme of the dynamic signaling events underlying TGF-β-induced changes in cell behavior.
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Mutations leading to a change in the Gibbs free energy (ΔΔG) < -1 kcal/mol or >1 kcal/mol were considered as non-neutral (i.e. both stabilizing and destabilizing mutations were taken as assays of change); all other mutations were treated as neutral (i.e. no effect).
As individuals taking ART may be misclassified on STARHS assays because of changes in HIV antibody level due to treatment, misclassification rates in this population may increase over time as more individuals initiate treatment[18].
These reaction conditions are subsaturating for ribosome binding in order to maximize the sensitivity of the assay for changes in activity due to the mutations.
Our laboratory work reflects the need for a relatively simple assay of parasite changes that may be occurring from praziquantel treatments.
For the external pH shift assays, the conditions were the same as the H2 production assays except that the assay was conducted for 12 minutes, and KOH or HCl was added 6 minutes after the start of the assay to change the external pH from 5.5 to 6.5 or from 6.5 to 5.5.
Because of changes in assay methodologies and different methods in associating the results with anthropometric outcomes, the magnitude of the association between the dual sugar permeability test and infant growth failure remains an open area of investigation.
Using proteasome inhibition assays, parallel changes of UPS activity at the protein level were determined in subcultured PBMCs (mainly composed of lymphocytes) from ALS patients, by Western blot analysis.
An alternative single end point screen might include assays that allow the detection of changes in insulin-regulated gene expression.
We also confirmed by immunoblot assay that the changes of the RLU were not due to changes of the amounts of the recombinant proteins accumulated in the transformed protoplasts (Figure 3B).
However, the potentials of SSH in assaying dynamic changes of gene expression in minute levels have never been addressed before.
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