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In plasmid shuffle assays (Materials and methods), the viability of cells containing the smc1-D1164E or SMC1 alleles was indistinguishable.
Enzymatic activities of CPM-labeled, e.g., Y274W-S96CCPM, and unlabeled Dpo4 mutants, e.g., Y274W-S96C, were measured under single-turnover conditions using P-based kinetic assays (Materials and Methods).
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Protein assay materials were purchased from Bio-Rad Laboratories (Hercules, CA).
To evaluate the effective of HSPG binding peptide in endothelial cell migration, we used rhVEGF165 to induce HUVECs migration in a transwell assay (Materials and Methods).
Protein assay materials and SYBR Green Supermix were purchased from Bio-Rad Laboratories (Hercules, CA), and Geneticin was from Life Technologies Corporation (Carlsbad, CA).
To evaluate the ability of apoEdp in blocking VEGF-induced HUVEC migration and invasion, we used a Boyden chamber transwell assay (Materials and Methods).
The binding and uptake of TfR was kinetically analyzed over 2 hrs with saturating levels of A568Tf (Figure 3A) in an approach to steady-state assay (Materials and Methods; [32]).
The authors thank BRAHMS (the manufacturer of procalcitonin assay) for providing assay materials.
All of the assay materials and reagents were provided by the Beckman Coulter Ins.
Label-free high-throughput screening methodologies such as differential scanning fluorimetry (DSF) forego the need to develop novel assay materials and/or reporters specific for individual protein targets.
To clarify further how IMPase regulates the synaptic polarity and consequently sensory behaviors, we conducted a genetic suppressor screen for ttx-7 mutants utilizing a population thermotaxis assay (Materials and Methods).
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