E.coli strain DH5α [genotype:F- supE44_lacU169 f80 lacZ_ M15 hsdR17 recA1 0endA1 gyrA96 thi-1 relA1] (Takara, Japan) was used for all DNA cloning assays in this study.
The water extracts of the compost was used for the germination assays in this study to overcome this effect.
The procedure of the behavioural assays in this study was adopted from that of Kasumyan and Morsi (1996) with modifications.
Finally, one gene belonging to the group of genes related with ribosomal proteins, RPS24, was selected as the housekeeping gene for the validation assays in this study.
The highly consistent results from the multiple qPCR assays and the low variances among the replicates clearly indicate that the qPCR assays in this study were robust, highly reproducible and the evaluation results are reliable.
We used human genomic DNA as the template in all digital PCR assays in this study.
The assays in this study where designed and conducted by JM.
The assays in this study were performed using entire gingival tissue, containing epithelial and connective tissue cells, including blood cells.
Concentrations of enzymes and substrates used in the enzyme assays in this study (Tables S1 and S2, respectively).
Due to the fact that the FIBTEM trace rarely reaches amplitude of 20 mm, the CFT and alpha angle was omitted for the FIBTEM assays in this study.
Both assays in this study have an excellent specificity and the sensitivity was 33% and 78% for the RVP and the RespiFinder-19 assay, respectively.
