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Therefore, we propose the use of both assays in the initial screening of RA in longitudinal studies, including early onset of undifferentiated arthritis.
Their study suggest that adding the assay of anti-MCV antibodies to the determination of anti-CCP2 increases the sensitivity for detecting seropositive RA, and authors propose the use of both assays in the initial screening of RA in longitudinal studies, including early onset of undifferentiated arthritis.
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Genotype imputation exploits known LD patterns and haplotype frequencies from reference data sets to estimate genotypes for additional SNPs not directly assayed in the initial genome-wide scan 45.
WJD assisted DOM in recruitment and conducting the study, carried out the betaine assays in the laboratory and made an initial attempt at analyzing and interpreting the results.PMG had overall supervision and clinical input and input into interpreting the results and edited the MS during preparation.
The assays used in the initial screen are listed in Table 1.
Two patients had antibodies; the assays used for antibody testing were different from the assay used in the initial phase III trial.
Base consumption as an assay of cephalosporin C conversion was validated by comparing the proton concentration (measured indirectly via the added NaOH) and 7-ACA (measured with a spectrophotometric assay) produced in the initial phase of the reaction.
Most of the annotations in the databases, however, are predicated on two key tenets in the "classical" approach in naming a gene or protein: (a) the assay used in the initial purification of the protein; and (b) localization in a subcellular compartment consistent with expectations.
For mitotic arrest assays, HeLa cells were treated with a 20.2-fold-titration (190pM to 10 μM) of each compound for 20 h and the half maximal inhibitory concentration (IC50) was derived for each compound using the cell cycle profile assay used in the initial screen.
The influence of such factors is easily addressed using competition assays which vary in the initial frequencies of altruists and cheats (Ross-Gillespie et al. 2007).
Peripheral blood mononuclear cells (PBMCs) from peripheral blood were isolated and ex vivo IFN-γ ELISPOT assays performed as described in the initial Phase 1 AMA-1/AS02A study previously conducted at WRAIR [16].
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