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Real time assays identified a significantly higher rate of mixed genotypes in Bangladeshi samples than the conventional gel-electrophoresis-based RT-PCR assay (32.5% versus 12.5%, P < 0.05).
The assays identified 58 potential ERK-primed GSK-3 substrates, of which 23 had evidence for in vivo phosphorylation in mass spectrometry databases.
In vivo transgenic reporter assays identified discrete tissue-specific regulatory modules within angptl4 intron 3 sufficient to drive expression in the liver, pancreatic islet β-cells, or intestinal enterocytes.
Luciferase-reporter and electrophoretic mobility shift assays identified the core responsive element of C/EBPβ in the p57Kip2 promoter between −150 and −130 bp region containing a putative C/EBP motif.
Microtubule assembly/disassembly and thermal aggregation assays identified the FI, LT, and ER peptides as interactive sequences in αB crystallin that were important for the dynamic assembly of microtubules.
Luciferase assays identified a strong activating element located at 5' promoter region, B, and two other activating elements within the 5' untranslated region (5'-UTR), E and F. A repressive fragment, as represented by elements C and D, is located between the two activating elements, B and E (S Fig. 2B).
The assays identified 13 miRNAs that inhibit AR expression.
Subsequent mobility shift and DNA footprinting assays identified a 30-bp ATAF2 binding sequence.
The PowerBlot and Kinexus assays identified a diverse range of proteins expressed in hESCs.
In vitro ubiquitination assays identified MuRF3 mono-ubiquitinated PPARα and PPARγ1, but not PPARβ.
These assays identified a significantly larger number of posterior midgut-enriched transcripts.
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