Sentence examples for assays for the initial from inspiring English sources
Genotyping assays for the initial screen were run on the Illumina BeadArray platform (www.illumina.com).
We used 2 types of assays for the initial viral screen, immunologic assays to identify antibodies and reverse transcription polymerase chain reaction (RT-PCR) to detect the viral genome.
We used in vitro Pol-β-directed strand-displacement activity assay for the initial screening of the compounds.
American Society of Clinical Oncology/College of American Pathologists (ASCO/CAP) guidelines recommend using either immunohistochemistry (IHC) assays for initial evaluation of HER-2 status followed by reflex testing by fluorescence in situ hybridisation (FISH) of some IHC categories or FISH in initial testing (Wolff et al, 2007).
For dephosphorylation assays, after the initial phosphorylation reaction described above, the DegS/YbdM and DegS/YabT reaction mixtures were desalted on a PD-10 column (to remove the ATP), lyophilized and resuspended in the identical reaction mixture as before, but without ATP, and incubated 2 hours at 37°C.
We retested the split-GAL4 drivers that showed a significant effect in the primary screening using a different dTrpA1 effector inserted at another genomic location, allowing us to assess expression more accurately since we had access to a reporter construct inserted at that site; these assays confirmed the initial results for all but one of the split-GAL4 driver lines.
The activity of these compounds as inhibitors of p53 MDM2 interaction was investigated using a yeast phenotypic assay, herein developed for the initial screening.
(A simplified version of this assay was employed for the initial measurements of Oct4 templates only.
For other assays the initial angle was calculated as the direction of movement over the first five frames.
As an initial validation, qPCR assays for 53 loci hypermethylated in at least 70% of tissue pairs were conducted across a panel of 16 independent IDCs (Stage II) and 25 normal or benign breast tissues.
