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A previously published dashboard application, the Toxcast Dashboard [99], also provides full access to single concentration assay data in the list of 'tested' assays endpoints if multi-concentration data are unavailable.
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When the cultured biomasses of all three Oscillatoria strains were extracted with 75%% aqueous methanol, the degree of inhibition by crude extracts of three different strains of Oscillatoria differed a lot against all tested enzymes in both the assays: endpoint and kinetic.
Results of the laboratory study showed that the comet assay endpoints of tail length and tail extent moment responded in a dose- and time-dependent manner.
A laboratory study exposing L. macrochirus to the known mutagen, methyl methanesulfonate, was performed to validate the comet assay endpoints in this species.
It is also crucial to recognize that the selection and use of techniques used to quantify assay endpoints (e.g., antibody concentration) will affect their accuracy and precision and these methods must also be defined and uniformly applied within and across laboratories.
A total of 624 in vitro assay endpoints ranging from gene to entire organism have been measured for each chemical in ToxCast Phase I.
The scaled activity values shown on the graph are calculated by dividing the response values by the activity cutoff, thereby enabling activity comparisons across assay endpoints.
The dichotomy of TPA-induced perturbations demonstrates that detecting the adverse effects of chemicals requires careful consideration of assay endpoints and experimental models.
To minimize variables in assay endpoints described below, control versus treated HRBECs were uniformly harvested from subconfluent populations at passages 25 27.
The molecualr genotoxicity assay endpoints from our assay were compared to those from conventional phenotypic genotoxicity assays, including alkaline comet assay in A549 cells (SI Figure S6) and other in vitro and in vivo assays from literature (Table 2).
In this case, therefore, the central conclusions of the study are based on multiple assay endpoints to test the effects of BPA with and without curcumin on three novel, spontaneously immortalized HRBEC lines.
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