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HPV types were determined from 2 commercial assays by using an algorithm which was evaluated earlier for this application (18, 19 ).
To probe the arrays, we performed different ChIP assays by using an antibody against SMAD4 in immortalized ovarian surface epithelial (IOSE) cells treated with TGF-β 1 for 0 and 3 hrs.
Bacterial DNA was extracted from 5 colonies by using 2% Chelex beads solution (Chelex 100 Resin; Bio-Rad, Richmond, CA, USA) and analyzed in 2 PCR assays by using an automated real-time thermocycler (Rotor Gene 6200HRM; Corbett Research, Mortlake, NSW, Australia).
cDNA was amplified in parallel assays by using an Applied Biosystems 7500 Fast Real-Time PCR System (Foster City, CA, USA) and incorporating primers and probes selective for the matrix gene of influenza A viruses, including that of the pandemic (H1N1) 2009 virus, and for the hemagglutinin (HA) gene of that virus.
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The concentrations of copeptin were assayed by using an immunofluorescent immunoassay (Copeptin; Brahms, Henningsdorf, Germany) with an automated Kriptor Analyzer (Thermo Fisher Scientific, Milan, Italy).
Lipase/esterase activity was assayed by using a spectrophotometer.
The luciferase and β-galactosidase activities were assayed by using a Luciferase assay kit (Promega, Madison, WI, USA).
As a further control, the localization of TPK1 in Arabidopsis cells was assayed by using a specific anti-TPK1 monoclonal antibody.
Activity was assayed by using a standard malachite green assay.
Intracellular cytokine was assayed by using a flow cytometer.
Hydroquinone hydroxylase and hydroxyquinol dioxygenase were assayed by using a fiber optic oxygen sensor.
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