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Differential CTC detection assays apply (or can be combined with) various strategies for the identification of tumor cells.
Notations: The standard assumptions of qPCR-based assays apply to the Fluidigm technology, namely that the cycle threshold (ct) is inversely proportional to the log of fluorescence.
However, it is essential that laboratories employing PCR-based assays apply very stringent laboratory quality control standards and that they validate these highly sensitive techniques following international guideline recommendations [ 37].
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A total of 27 samples were analysed using the ABTS and FRAP assays applied to the soluble and insoluble fractions.
Moreover, other qrt-PCR assays applied to environmental samples have yielded highly variable results.
Beads containing carboxyl groups with a density of 160 185 µEq/g were found to be optimal for assays applied to a wide range of cell types.
The common assays applied to quantify SHR rely on the precise restoration of transgenic marker genes from two incomplete but overlapping parts [9], [14].
Both assays applied were thoroughly validated prior to analysis of plasma samples.
In the present study, we propose a guideline to validate qPCR assays applied to food microbiology.
Xenotransplantation assays applied to breast cancer may only identify cells capable of engraftment in particularly permissive (micro environments.
In the present study, we propose a strategy to evaluate the performance of qPCR assays applied to mold detection.
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